Turfgrass Science Team at the 2019 ASA-CSSA-SSSA Annual Meeting

December 4, 2019

The Turfgrass Science team from the University of Minnesota was well-represented at this year’s ASA-CSSA-SSSA Annual Meeting held on November 10-13, 2019 in San Antonio, TX.  Seven people gave oral presentations and five people presented posters. Additionally, congratulations go to two members of our group who placed in the graduate student oral presentation contests:

  • Garett Heineck was awarded 2nd place for his oral presentation in the Genetics and Molecular Techniques section of the C5 Division.
  • Ryan Schwab was awarded 3rd place for his oral presentation in the Golf Turf section of the C5 Division.

Below is a listing of all the oral presentations and posters along with links to the presentation abstracts and the poster PDF files.

Oral presentations

Increasing Competition in Perennial Ryegrass Selection Nurseries Bolsters Prediction Accuracy

Presented by Garett Heineck; co-authors Nancy Jo Ehlke, Kayla Altendorf and Eric Watkins

The success of a turf-type perennial ryegrass variety depends on both adequate turfgrass quality and economic seed yield. In most breeding programs, spaced plants are the initial unit of selection wherein observations of related individuals dictate selections of superior germplasm for further testing. As such, spaced plants must be predictive of both sward environments. This research explored the effectiveness of both standard (3 plants m-2) and competitive (23 plants m-2) spaced plant nurseries as selection environments using 20 turf-type entries. Seed production plots, turfgrass plots, and the two spaced-plant growing environments were tested at two locations in Minnesota in 2017 and 2018. Meaningful breeding targets for seed production were discovered using structural equation modeling (SEM). Traits important for superior turfgrass quality were determined through multiple regression across many rating dates. Results showed that winter survival was imperative to obtain optimum seed yield and turfgrass quality, albeit only to a certain extent. Seed yield was only affected when tiller survival was below 20% whereas turfgrass quality was impacted when tiller survival was much higher (<60%). The seed yield components fertile tiller number, spike yield, and fertility index (seed fill efficiency) were identified by the SEM as important breeding targets. Of these, fertility index in spaced plants was by far the most useful indicator of sward yield, especially in the competitive nursery, where rank correlations were substantial at both locations (rs = 0.52 and 0.81, P < 0.05). For turfgrass traits, genetic color and crown rust were the most important predictors of turfgrass quality. In this case, the competitive spaced plant design offered minor improvements of predictive ability compared to the standard environment. Overall, increased competition between spaced plants improved the predictive ability (rs) for seed production traits and while it offered only a small benefit for most turfgrass traits, it did not decrease predictive ability. Furthermore, the competitive design has a lower field space requirement and facilitates more efficient data collection for many traits compared to a standard design.

Wetting Agent Influence on Soil Water Repellency Parameters at Different Rootzone Temperatures

Presented by Ryan Schwab; co-authors Brian Horgan and Samuel Bauer

Wetting agents are commonly applied to golf turf to solve moisture issues. They may alter water retention in the rootzone as well as prevent localized dry spots by reducing the degree and persistence of soil water repellency. Reapplication is needed to maintain these functions; however, it is unclear how much environmental factors such as soil temperature and water volume from irrigation or rain events influence an ideal reapplication interval. The objective of this research was to test if a wetting agent, Cascade Plus, lost function when exposed to different soil temperatures over time. Creeping bentgrass (Agrostis stolonifera) plugs in conetainers were maintained at three soil temperature treatments (21.5, 28, and 34.5 °C) within porous heat baths. Water retention, water droplet penetration time (WDPT), critical moisture content, and the area below the WDPT-soil moisture curve were quantified 10, 20, and 30 days after a single Cascade Plus application. These soil water repellency parameters were compared across time points and to an untreated control. Preliminary results suggest that Cascade Plus may reduce all of the quantified soil water repellency parameters at all soil temperature exposure levels compared to an untreated control, and water retention measured gravimetrically was not a useful function to determine its efficacy. Results from this work will lead to a clearer understanding of environmental influence on wetting agent functions for more efficient reapplication programs.

Improving Our Approach on How We Analyze Turfgrasses for Tolerance to Foliar Shade

Presented by Dominic Petrella; co-author Eric Watkins

Turfgrass is often affected by foliar shade such as that from trees and shrubs. This type of shade results in reductions in light quantity and the ratio of red to far-red light (R:FR), both of which can decrease turfgrass quality. The conditions produced by foliar shade are quite variable in nature, and little to no information is available on common reductions in light intensity or commonly found R:FR ratios. A considerable amount of research has evaluated the effects of reduced light quantity on turfgrasses using neutral shade cloth and arbitrary reductions in light quantity; however, most of these studies have not addressed changes in R:FR. Experiments have been performed under real-world foliar shade, but a review of the literature indicated that light quantity is not always monitored and the R:FR ratio is rarely reported. The objective of this research was to evaluate changes in light quantity and the R:FR ratio under foliar shaded turfgrasses in St. Paul MN to better understand how we can approach improving tolerance to foliar shade. The results of a two year study indicate that we may be able to better understand foliar shade sites by 1) taking into account fluctuations in light intensity by collecting data every one minute, 2) examining the number of hours at given light intensities rather than only evaluating the daily light integral, and 3) monitoring R:FR in morning, solar noon, and in the evening.

Fine Fescues As Living Mulch in Minnesota Cropping Systems

Presented by Florence Sessoms; co-authors Eric Watkins, M. Scott Wells, Nancy Jo Ehlke, Dominic P. Petrella, Donn Vellekson and Alexander Hard

Intercropping systems are thought to improve the use of water and nutrients while decreasing pests. Turfgrasses are known for their ability to reduce soil erosion and nutrient run-off in urban ecosystems, and previous research has evaluated their use in agronomic intercropping. Fines fescue (Festuca spp.) turfgrasses have the ability to reduce weed incidence in field experiments, and could be suitable as living mulch in strip intercropping systems; However, few studies have evaluated these species as a companion crops. We tested the presence of hard fescue (Festuca brevipila), Chewings fescue (Festuca rubra ssp. commutata), and strong creeping red fescue (Festuca rubra ssp. rubra) as companion crops with wheat, flax and barley for fine fescue seed production. All experiments were planted using strip intercropping in St. Paul or Roseau MN in 2017 and 2018. Strip intercropping provokes a reduction of light quantity and quality for the lower canopy. In our experiments, all cash crops decreased light quantity and light quality near the turfgrass which reduced establishment and seed yield for all fine fescue species. Overall, hard fescue showed the greatest yield reduction in wheat while Chewings fescue displayed a reduced yield loss. Our results demonstrate that using fine fescue species as a companion crop in strip intercropping systems can be done, but more research is needed to improve their shade tolerance in order to improve establishment and increase seed yield in this system. Nutrient competition between the two crops could also occur, leading to yield losses for the cash crop. We have not observed a negative effect on wheat yield when grown with fine fescues, indicating that species-specific intercropping has future potential.

Unveiling Transcriptome Composition in Hexaploid Hard Fescue (Festuca brevipila) through Pacbio Isoform Sequencing

Presented by Yinjie Qiu; co-authors Cory Hirsch and Eric Watkins

Hard fescue (Festuca brevipila Tracey, 2n=6x=42) is a fine-textured, cool-season turfgrass that does well under low-input management. Breeding and genetics studies of F. brevipila have been limited due to the complex nature of its hexaploid genome. To advance our knowledge of F. brevipila, we used PacBio isoform sequencing to develop a reference transcriptome of this taxon. Here, we report the F. brevipila reference transcriptome generated from root, crown, leaf, and seed head tissues using 4 SMRT cells. We obtained 59,510 full-length transcripts and further refined them into 38,595 non-redundant full-length transcripts which had a N50 of 2,585 bp. The longest and shortest transcripts were 11,487 and 58 bp, respectively. Transcriptome annotation using NCBI NR protein and Uniprot databases returned 36,075 (93.47%) and 29,670 (76.88%) annotated transcripts, respectively. Finally, we reconstructed the cytochrome P450 gene family phylogenetic tree of F. brevipila to study the effect of propiconazole fungicide on this complex gene family. We found gene expression of CYP709B3 and CYP94C1 had been affected 6 days post the propiconazole treatment. We also identified several gene expression changes in the fatty acid elongation pathway and genes related to histone modifications. Overall, this F. brevipila reference transcriptome will provide geneticists with a foundation to further study this important turfgrass taxon.

Assessment of Tillering and Rhizomatous Growth in Strong Creeping Red Fescue

Presented by Nicole Mihelich; co-authors Dominic P. Petrella, Florence Sessoms, Laura M. Shannon and Eric Watkins

Tillers and rhizomes are important physiological feature for turfgrasses, and thus may be traits deserving of more focus for cool-season turfgrass breeding. These two types of stems can allow for resilience and competitiveness in a lawn. Tillers and rhizomes are also thought to be helpful for sod production. Kentucky bluegrass has the ability to form rhizomes, and is the predominant sod species in Minnesota and the surrounding states; however, consumers would benefit from increased availability of fine fescue sod for lawns, roadsides, and other areas where low-input species would be useful. Strong creeping red fescue (Festuca rubra ssp. rubra) displays robust rhizomatous growth that parallels Kentucky bluegrass. The investigation of tillering and rhizome traits of this fine fescue species could help plant breeders develop new cultivars with enhanced sod forming ability. The objective of this project was to quantify tiller and rhizome production in a diverse set of strong creeping red fescue germplasm. A total of 192 accessions were each represented by single genotypes in each of the four replications; we also included check cultivars of Kentucky bluegrass, strong creeping red fescue, hard fescue, and Chewings fescue. One-month-old plants, started from seed, were grown under Minnesota summer conditions for three months. Non-destructive aboveground phenotypic data was taken every two weeks for vertical growth, tillering, and daughter plant production. After three months, destructive phenotypic data was taken to quantify and characterize belowground rhizomatous growth. The panel revealed high variation in the measured traits. Tiller number ranged from 2 to 115, and rhizome number ranged from 0 to 23. The majority of accessions were capable of rhizome formation, which appeared to accelerate in the final month of the study. This diversity should be useful for turfgrass breeders in the development of new cultivars of fine fescue for use in sod production systems.

Natural Weed Suppression of Crabgrass Varies By Genotype and Field Management Practices

Presented by Jon Trappe; co-authors Eric Watkins, Dominic P. Petrella and Florence Sessoms

Chewings fescue (Festuca rubra ssp. commutata) has shown potential for natural suppression of crabgrass (Digitaria spp.). Previous work has identified differences among cultivars and species of fine fescues (Festuca ssp) for the amount of crabgrass suppressed. Other work has shown that nutrient status may play an important role in regulating suppression in Chewings fescues. More research is needed to better understand factors controlling variability of crabgrass suppression within Chewings fescue, and how management practices such as mowing and fertilization affect suppression in the field. Therefore the objectives of this experiment were to 1) examine whether weed suppression varies by genotype within an accession or cultivar, and to 2) determine the effect of mowing frequency and nitrogen fertility on allelopathic potential of ‘Radar’ Chewings fescue and ‘Beacon’ hard fescue. For objective 1, 12 genotypes each for a pre-determined low, medium, and highly suppressive Chewings fescue accessions or cultivar were examined for their variability in crabgrass suppression by measuring a reduction in adjacently grown crabgrass biomass within pots in a greenhouse experiment. For objective 2, a split-strip plot design was used in the field to examine the effect of two mowing frequencies (once per week or 3 days per week) stripped across whole plots and two nitrogen fertility (no nitrogen or 46 kg N ha-1 yr-1) split across whole plots have on crabgrass suppression by ‘Radar’ Chewings fescue and ‘Beacon’ hard fescue. Preliminary results indicate that crabgrass suppression varies by genotype within an accession of Chewings fescue and that field management practices may have an impact on crabgrass growth in the field.

Posters

Environmental Impacts of Smart Irrigation and Mowing Height in Kentucky Bluegrass Lawns

By Daniel Sandor; co-authors Brian Horgan and Brian Davis

University Digital Conservancy link to poster

Precision Irrigation for Golf Courses Using Sensor and Mapping Technologies

By Chase Straw; co-authors Joshua Friell and Brian Horgan

University Digital Conservancy link to poster

Minnesota Regional Roadside Seed Bank Analysis

By Dominic Christensen; co-authors Joshua Friell, Jacob Jungers, Jon M. Trappe and Eric Watkins

University Digital Conservancy link to poster

Optimum Seeding Rate and Biomass Removal Timing for No-Mow Fine Fescue Golf Course Roughs

By Ryan Schwab; co-authors Eric Watkins, Andrew Hollman, Brian Horgan and Samuel Bauer

University Digital Conservancy link to poster

Developing Online Education and Training for Installation and Management of Roadside Turfgrasses

By Kristine Moncada; co-authors Jon M. Trappe, Samuel Bauer and Eric Watkins

University Digital Conservancy link to poster